Sains Malaysiana 33(1): 63-72 (2004) Sains Hayat /
Life Science
Penghasilan Hidrolisat Protein Kuning
Telur Melalui Hidrolisis Berasid
(Production of egg yolk protein hydrolysate
through acid hydrolysis)
Wan Aida Wan Mustapha & Su Koh Shin
Program Sains Makanan
Pusat Pengajian Sains Kimia dan Teknologi Makanan
Fakulti Sains dan Teknologi
Universiti Kebangsaan Malaysia
43600 UKM Bangi Selangor D.E., Malaysia
ABSTRAK
Kandungan kuning telur luar gred yang melebihi 50% daripada telur biasa didapati sesuai untuk digunakan dalam pemprosesan perisa hidrolisat. Pengekstrakan protein dilakukan dengan mengguna etanol dan air pada dua nisbah berbeza iaitu 1:1:1 dan 1:5:2 (kuning telur : etanol : air). Protein kuning telur di hidrolisis menggunakan 3N dan 5N asid hidroklorik selama 10 jam pada suhu 110oC. Hasil akhir hidrolisat protein dikeringkan secara semburan sejuk-beku. Hasil kajian mendapati pengekstraksian menggunakan nisbah 1:5:2 memberikan kandungan protein yang paling tinggi. Hidrolisat protein dengan perlakuan 5N asid hidroklorik mempunyai darjah hidrolisis yang paling tinggi. Sampel gabungan 1:5:2 - 5N memberikan darjah hidrolisis tertinggi (78.3%) diikuti oleh gabungan 1:1:1 -5N (69.4%) dan 1:5:2 - 3N (66.9%). Hasil penilaian sensori menunjukkan sampel 5:2 - 3N memberikan kekuatan aroma yang tertinggi di antara keempat-empat sampel. Sebatian aroma yang dihasilkan daripada hidrolisat kuning telur lebih menyerupai pati ayam Brands atau ekstrak lembu Brovil di mana kehadiran sebatian-sebatian kumpulan amina, alkohol, alkana, indol dan fenol telah dikesan menggunakan Kromatografi Gas ruang tutupan.
ABSTRACT
Off grade egg with egg yolk content of 50% higher than normal egg can be successfully used in processing of hydrolysate flavourings. Protein extraction using ethanol with two different ratios of egg yolk: ethanol: water ( 1: 1: 1 and 1:5:2) was carried out. The egg yolk protein was hydrolysed with 3N and 5N HCI for 10 hours at 11 ()'C. The protein hydrolysate obtained was freeze dried. Studies showed that extraction using 1:5:2 ratio gave the highest protein content. The protein hydrolysate treated with 5N HCI gave the highest degree of hydrolysis. Sample of 1:5:2 - 5N had the highest degree of hydrolysis (78.8%),followed by sample of 1:1:1-5N (69.4%) and 1:5:2 - 3N (66.9%). Sensory evaluation results showed that sample of 1:5:2 - 3N produced the strongest aroma among the four samples studied. Aroma of egg yolk protein hydrolysate was similar to Brands Chicken Essence or Brovil Beef Extract. The presence of volatile compounds with amine chemical groups, alcohol, alkane, indole and phenol were detected using headspace Gas Chromatography.
RUJUKAN/REFERENCES
Adler-Nissen, J. 1986. Enzymic Hydrolysis of Food Protein. London: Elsevier Applied Science.
Anon. 1984. Official Methods of Analysis of The Association of The Association Analytical Chemists. Ed. Ke-15. Arlington: AOAC Publishers.
Becker, W.A., Spencer, J.V., Verstate, J.A. & Mirosh, L.W. 1977. Genetic Analysis of Chicken Egg Yolk Cholesterol. Poultry Science 56:895-901.
Gordon, I. 1996. Market report: Easy vs West. Food Ingredient & Analysis International 18(4):21-23.
Hino, T., Nakanishi, S. & Hobo, T. 1996. Development of Whole Headspace Injection Method for the Determination of Volatile Organic Compound in H2O. Journal Chromatography 46:83-90.
Hoyle, N. T. & Merritt, J. H. 1994. Quality of Fish Protein Hydrolisates from Herring (Clupea harengus). Journal of Food Science 59( I ):76.
Larsen, J.E. & Froning, G.W. 1981. Extraction and Processing of Various Components from Egg Yolk. Poult. Sci. 60: 160-167.
Li-Chan, C.Y., Powrie, W.O. & Nakai, S. 1986. The Chemistry of Eggs and Egg Products. Dim. Stadelman, WJ. & Cotterrill, OJ. (pnyt). Egg Science and Technology, Ed. ke 4, Westport: Avi Publishing Co.
Lunven, P., Le Clement De St Marcq, C., Carnovale, E. & Fratone, A. 1973. Amino Acid Composition of Hen Egg. British Journal of Nutrition 30(2): 189-194.
Mottram, D.S. 1991. Meat. Dim. Volatile Compounds in Foods and Beverages. Maarse, H. (pnyt.), New York: Marcel Dekker Inc.
Mottram, D.S. & Whitfield, F.B. 1995. Maillard Lipid Interaction in Nonaqueous System: Volatile from the Reaction of Cysteine and Ribose with Phosphatidycholine. Journal of Agriculture and Food Chemistry 43: 1302-1306.
Powrie, W.O. 1977. Chemistry of Egg and Egg Products. Dlm. Egg Science and Technology. Stadelman, W.1. & Cotterri II , 0.1. (pnyt.). Ed. ke 2, Conneticutt: A VI Publishers Co.
Warren, M.W., Larick, O.K. & Ball, H.R. 1995. Volatile and Sensory Characteristic of Cooked Egg Yolk, White and Their Combinations. Journal of Food Science 60(1):79-84.
Wattanapat, R., Nakayama, T., Beuchat, L.R. & Phillips, R.D. 1994. Kinetics of Acid Hydrolysis of Defatted Peanut Flour. Journal of Food Science 59(3): 621.
Weir, G.S.D. 1992. Proteins as a Source of Flavour. Dlm. Biochemistry of Food Protein, Hudson, B.J.F. (pnyt.). London: Elsevier Science Publisher Ltd.
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