Sains Malaysiana 47(4)(2018): 661-670

http://dx.doi.org/10.17576/jsm-2018-4704-03

 

5-Aminolevulinic Acid Induces Regulation in Growth, Yield and Physio-Biochemical Characteristics of Wheat under Water Stress

(Asid 5-aminolevulinic Mengaruh Pengawalaturan terhadap Pertumbuhan, Hasilan dan Ciri Fisio-Biokimia Gandum pada Tekanan Air)

 

NUDRAT AISHA AKRAM1*, SHAMIM KAUSAR1, NAILA FARID1, MUHAMMAD ASHRAF2,3 & FAHAD AL-QURAINY3

 

1Department of Botany, Government College University Faisalabad Pakistan

 

2Pakistan Science Foundation Islamabad, Pakistan

 

3Department of Botany and Microbiology King Saud University Riyadh, Saudi Arabia

 

Diserahkan: 23 Mei 2017/Diterima: 30 Oktober 2017

 

 

ABSTRACT

 

The production of wheat crop is below average in many regions of the world which is ascribed to adverse environmental conditions including drought stress. The present study was conducted to appraise the beneficial role of exogenously- applied 5-aminolevulinic acid (ALA) on growth, yield and some key physio-biochemical characteristics of two commercially important wheat cultivars (Shafaq-06 and Uqab-2000) under well watered [100% field capacity (FC)] and water-deficit (60 and 80% FC) conditions. Imposition of varying water regimes significantly decreased fresh and dry weights of shoots, photosynthetic pigments (a and b), non-photochemical quenching of chlorophyll fluorescence (NPQ), quenching coefficient for non-photochemical (N) of chlorophyll fluorescence (qN), K+ (potassium ion), Ca2+ (Calcium ion) and P (phosphorus) accumulation in shoot and root and yield-related attributes. In contrast, water deficit regimes caused improvement in Fv/Fm (chlorophyll fluorescence measurement), coefficient of photochemical quenching (qP), proline, glycinebetaine (GB) and hydrogen peroxide (H2O2) contents. Foliar spray of ALA at the rate of 50, 100 and 150 mg/L along with control (no spray (NS) and/or water spray (WS)) significantly enhanced chlorophyll a and b pigments, qN, NPQ, qP, K+, Ca2+ and P accumulation in both roots and shoots, proline, GB, total phenolics and malondialdehyde (MDA) contents and yield. The wheat Shafaq-06 was better in shoot dry weight, qN, NPQ and Fv/Fm, shoot and root K+, root Ca2+, proline, GB accumulation and yield attributes, while Uqab-2000 was better in chlorophyll a contents, root P and MDA contents. Overall, better growth and yield of Shafaq-06 than Uqab-2000 under water deficit regimes was found to be associated with ALA improved leaf fluorescence (qN, NPQ and Fv/Fm), shoot and root K+, root Ca2+, proline and GB accumulation.

 

Keywords: Chlorophyll contents; drought stress; wheat; yield; 5-Aminolevulinic acid

 

 

ABSTRAK

 

Penghasilan tanaman gandum adalah di bawah purata di kebanyakan kawasan di dunia dianggap sebagai keadaan alam sekitar yang buruk termasuk tekanan kemarau. Kajian ini dijalankan untuk menilai kelebihan eksogen-aplikasi 5-aminolevulinik asid (ALA) terhadap pertumbuhan, hasilan dan ciri-ciri fisio-biokimia kedua-dua kultivar gandum yang komersial utama (Shafaq-06 dan Uqab-2000) dalam keadaan kecukupan air [100% keupayaan lading (FC)]  dan defisit air (60 dan 80% FC). Pengenaan rejim air yang berbeza telah mengurangkan berat segar dan kering tunas secara berkesan, pigmen fotosintetik (a dan b), pelindapan tanpa fotokimia fluoresens klorofil (NPQ), pekali pelindapan untuk tanpa fotokimia (N) fluoresens klorofil (qN), K+ (ion kalium), Ca2+ (ion kalsium) dan P (fosforus) pengumpulan pada tunas, akar serta sifat berkaitan hasilan. Sebaliknya, rejim defisit air menyebabkan penambahbaikan Fv/Fm (pengukuran fluoresens klorofil), pekali pelindapan fotokimia (qP), kandungan prolina, glycinebetaine (GB) dan hidrogen peroksida (H2O2). Semburan daun ALA pada kadar 50, 100 dan 150 mg/L selari dengan kawalan (tanpa semburan (NS) dan semburan air (WS)) meningkat dengan ketara pengumpulan pigmen klorofil a dan b, qN, NPQ, qP, K+, Ca2+ dan P pada akar dan tunas, serta kandungan dan hasilan prolina, GB, jumlah fenolik dan malondialdehid (MDA). Gandum Shafaq-06 lebih baik dalam berat kering tunas, qN, NPQ dan Fv/Fm, tunas dan akar K+, tunas dan akar Ca2+, prolina, pengumpulan GB dan sifat hasilan, manakala Uqab-2000 lebih baik dalam kandungan klorofil a, tunas P dan kandungan MDA. Keseluruhannya, pertumbuhan dan hasilan Shafaq-06 lebih baik daripada Uqab-2000 dalam regim defisit air adalah berkaitan dengan ALA yang dapat menambahbaik flurosens daun (qN, NPQ, dan Fv/Fm), tunas dan akar K+, akar Ca2+, prolina dan pengumpulan GB.

 

Kata kunci: Asid 5-aminolevulinik; gandum; hasilan; kandungan klorofil; tekanan kemarau

 

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*Corresponding author; email: nudrataauaf@yahoo.com

 

 

 

 

 
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