Sains Malaysiana 47(7)(2018): 1535–1540

http://dx.doi.org/10.17576/jsm-2018-4707-22

 

Comparison of DNA Profiling between Fishes and Pork Meat using Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCR-RFLP) Analysis

(Perbandingan Profil DNA antara Ikan dan Daging Babi menggunakan Analisis Tindak Balas

Rantaian Polimerase-Polimorfisme Panjang Cebisan Pemotongan (PCR-RFLP))

 

SAFIYYAH SHAHIMI, SAHILAH ABD. MUTALIB*, WAN SAKEENAH WAN NAZRI, MINAH ABDULLAH & NORRAKIAH ABDULLAH SANI

 

School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 14 September 2017/Diterima: 23 Februari 2018

 

ABSTRACT

 

Genomic DNA of 13 fish (n=13) species consist of four freshwater which were catfish (Clarias gariepinus), shark catfish (Pangasius larnaudii), tilapia (Oreochromis mossambicus), perch (Lates calcarifer) and nine marine species which were black pomfret (Parastromateus niger), anchovy (Stolephorus commersonii), mabong (Rastrelliger kanagurta), red snapper (Lutjanus erythropterus), herring (Chirocentrus dorab), ray fish (Himantura gerrardii), sardine (Decapterus macrosoma), mackerel (Euthynnus affinis) and tuna (Thunnus tuna) were differentiated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Seven endonucleases of AluI, BsaJI, HaeIII, HindIII, HinfI, MboI and MboII were examined for the ability to digest cyt b amplicon from each species. Genomic DNA of pork (Sus scrofa domestica) were differentiated from fishes by comparing the digestion patterns produced by similar amplified region and enzymes used. In the present study, it was demonstrated that fishes and pork DNA genome were successfully differentiated using all endonucleases except for HindIII. Thus, PCR-RFLP analysis was found useful for future pork DNA detection in fish products.

 

Keywords: DNA profiling; fish; PCR-RFLP; pork (Sus scrofa domestica)

 

ABSTRAK

DNA genomik 13 spesies ikan (n=13) terdiri daripada empat ikan air tawar iaitu keli (Clarias gariepinus), patin (Pangasius larnaudii), tilapia (Oreochromis mossambicus), siakap (Lates calcarifer) dan sembilan ikan air masin iaitu bawal hitam (Parastromateus niger), ikan bilis (Stolephorus commersonii), mabong (Rastrelliger kanagurta), ikan merah (Lutjanus erythropterus), ikan parang (Chirocentrus dorab), pari (Himantura gerrardii), sardin (Decapterus macrosoma), tenggiri (Euthynnus affinis) dan tuna (Thunnus tuna) telah dibezakan menggunakan tindak balas rantaian polimerase-polimorfisme panjang cebisan pemotongan (PCR-RFLP). Tujuh endonuklease iaitu AluI, BsaJI, HaeIII, HindIII, HinfI, MboI dan MboII telah dinilai kebolehannya bagi mencernakan amplikon cyt b daripada setiap spesies. DNA genomik babi (Sus scrofa domestica) telah dibezakan daripada ikan dengan membandingkan corak pencernaan yang terhasil ke atas kawasan amplifikasi yang serupa menggunakan enzim yang digunakan. Dalam kajian ini, DNA genomik ikan dan babi telah berjaya dibezakan oleh kesemua endonuklease kecuali enzim HindIII.

 

Kata kunci: Babi (Sus scrofa domestica); ikan; PCR-RFLP; pemprofilan DNA

 

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*Pengarang untuk surat-menyurat; email: sahilah@ukm.edu.my