Sains Malaysiana 50(6)(2021): 1745-1754
http://doi.org/10.17576/jsm-2021-5006-20
The Effects of Apitherapeutic Agents on
Oxidative Stress in Serum Metabolic Parameters of Hypertensive Rats Created by
Nitric Oxide Synthase Inhibited
(Kesan Agen Apiterapeutik terhadap Tegasan Oksidaan dalam Parameter Metabolisme Serum Tikus Hipertensi dihasilkan oleh Sintase Terencat Oksida Nitrik)
MEHMET
FUAT GULHAN1, BETUL OZDEMIR2*, ZELIHA SELAMOGLU3 &
ENGIN SAHNA4
1Department
of Medicinal and Aromatic Plants, Vocational School of Technical Sciences, Aksaray University, Aksaray, Turkey
2Department
of Cardiology, Faculty of Medicine, Nigde Ömer Halisdemir University, Campus, Nigde, Turkey
3Department
of Medical Biology, Faculty of Medicine, Nigde Ömer Halisdemir University, Campus, Nigde, Turkey
4Department
of Pharmacology, Faculty of Medicine, Firat University, Elazig, Turkey
Diserahkan: 7 Ogos 2020/Diterima:
30 November 2020
ABSTRACT
Hypertension is a chronic disease affecting the whole
world due to its clinical effects and complications. The reduction in the
release and effect of NO is one of the mechanisms of hypertension formation.
Hypertension disrupts the balance between oxidant and antioxidant mechanisms.
In this study, we aimed to observe biochemical changes in the blood by
treatment propolis, caffeic acid phenethyl ester (CAPE) and pollen in
hypertensive rats via Nω-Nitro-L-arginine methyl
ester (L-NAME). Rats were divided in five groups. Rats were given L-NAME (40
mg/kg, intraperitoneally) for 14 days to make hypertensive. L-NAME and bee
products were administered together with rats for 14 days, then L-NAME for 14
days. All administrated ended 28 days. There was a statistically significant
(P<0.05) decrease in blood pressure (BP) in the groups in which bee products
were applied. Blood pressure was lower in the pollen treated group than in the
CAPE and propolis treated group (P<0.05). Paraoxanase (PON1), total antioxidant status (TAS), total oxidant status (TOS), asymmetric
dimethylarginine (ADMA), nuclear factor-κB (NF-κB) levels were measured in the blood samples in all
groups. In the L-NAME group; PON1, TAS, HDL, and total protein levels were
found to be lower than the groups applied bee products (P<0.05). TOS,
oxidative stress index (OSI), ADMA, NF-κB,
glucose, cholesterol, LDL, triglyceride, ALT, AST, ALP levels were found to be
lower in groups plus of bee products were applied compared to the group that
received L-NAME (P<0.05). The results showed that oxidative stress and
homeostasis can be regulated with propolis, CAPE and pollen in hypertensive
rats induced L-NAME.
Keywords: Asymmetric dimethylarginine; bee products;
blood; nuclear factor-κB; oxidative stress; paraoxanase; rat
ABSTRAK
Hipertensi
adalah penyakit kronik yang menyerang seluruh dunia kerana kesan dan komplikasi
klinikalnya. Pengurangan pembebasan dan kesan NO adalah salah satu mekanisme
pembentukan hipertensi. Hipertensi mengganggu keseimbangan antara mekanisme
oksidan dan antioksidan. Tujuan kajian ini adalah untuk memerhatikan
perubahan biokimia dalam darah dengan propolis rawatan, asid fenil ester kafeik
(CAPE) dan debunga pada tikus hipertensi melalui metil ester
Nω-Nitro-L-arginine (L-NAME). Tikus dibahagikan dalam lima kumpulan. Tikus
diberi L-NAME (40 mg/kg, intraperitoneal)
selama 14 hari untuk membuat hipertensi. L-NAME dan produk lebah diberikan
bersama tikus selama 14 hari, kemudian L-NAME selama 14 hari. Semua pemberian berakhir
pada 28 hari. Terdapat penurunan tekanan darah (P <0.05) yang signifikan
secara statistik pada kelompok yang menggunakan produk lebah. Tekanan darah
lebih rendah pada kumpulan yang dirawat debunga daripada kumpulan yang dirawat
CAPE dan propolis (P <0.05). Paraoksanase (PON1), status antioksidan total
(TAS), status oksidan total (TOS), dimetilarginin asimetrik (ADMA), tahap
faktor nuklear-κB (NF-κB) diukur dalam sampel darah dalam semua
kumpulan. Dalam kumpulan L-NAME; Tahap protein PON1, TAS, HDL dan jumlah protein
didapati lebih rendah daripada kumpulan produk lebah yang digunakan (P
<0.05). TOS, indeks tekanan oksidatif (OSI), ADMA, NF-κB, glukosa,
kolesterol, LDL, trigliserida, ALT, AST, kadar ALP didapati lebih rendah dalam
kumpulan ditambah produk lebah digunakan berbanding dengan kumpulan yang
menerima L- NAMA (P <0.05). Hasil kajian menunjukkan bahawa tekanan
oksidatif dan homeostasis dapat diatur dengan propolis, CAPE dan debunga pada
tikus hipertensi yang disebabkan L-NAME.
Kata kunci: Darah; dimetilarginina tak simetri; faktor nuklear-κB; paraoksanase; produk lebah; tegasan oksidaan; tikus
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*Pengarang untuk surat-menyurat; email: betulozaltun@ohu.edu.tr
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