Sains Malaysiana 33(1): 73-81 (2004) Sains Bumi /
Earth Science
Penghasilan Aseton-Butanol-Etanol dan Gas Hidrogen daripada
Efluen Kilang Kelapa Sawit Secara Fermentasi Anaerobik
dengan Clostridium acetobutylicum NCIMB 13357
(Production of acetone-butanol-ethanol and hydrogen of palm oil mill effluent
anaerobic fermentation with Clostridium acetobutylicum NCIMB 13357)
Pang Wey Kit, Wan Mohtar Wan Yusoff
Pusat Pengajian BioSains dan Bioteknologi
Fakulti Sains dan Teknologi
Universiti Kebangsaan Malaysia
43600 UKM Bangi Selangor D.E. Malaysia
Mohd Sahaid Kalil
Jabatan Kejuruteraan Kimia dan Proses
Fakulti Kejuruteraan
Universiti Kebangsaan Malaysia
43600 UKM Bangi, Selangor D.E.Malayisa
Osman Hassan
Pusat Pengajian Sains Kimia dan Teknologi Makanan
Fakulti Sains dan Teknologi
Universiti Kebangsaan Malaysia
43600 UKM Bangi, Selangor D.E. Malaysia
ABSTRAK
Kajian ke atas penggunaan secara terus efluen kilang sawit (POME) sebagai medium fermentasi aseton-butanol-etanol dan hidrogen (ABE/H) oleh Clostridium acetobutylicum NClMB 13357 telah dilakukan dalam sistem kultur kelompok. Kesan kepekatan POME termendak dan kesan pH awal medium ke atas penghasilan ABE/H telah diteliti. Didapati 90% POME termendak (POME90) menghasilkan jumlah kepekatan ABE yang tertinggi sebanyak 4.01 g/L (pH 5.8) dengan kepekatan aseton; 1. 97g/L, butanol; 1.74g/L dan etanol; 0.3g/L. Penghasilan butanol adalah maksimum pada pH 6.0 iaitu sebanyak 1.86g/L. Kultur dengan kepekatan POME90 menghasilkan gas hidrogen lebih tinggi berbanding dengan kultur berkepekatan POME termendak yang lebih rendah. Penghasilan gas hidrogen didapati tertinggi pada pH 5.5 (31mL). Gas hidrogen turut dihasilkan pada pH awal medium lain dengan penghasilan melebihi 50% (v/v) kecuali pada pH 4.5 yang hanya menghasilkan 5mL (16% v/v) gas hidrogen.
ABSTRACT
Palm oil mill effluent (POME) can be utilised directly as the sole substrate in the anaerobic fermentation of acetone-butanol-ethanol (ABE) and hydrogen by Clostridium acetobutylicum NClMB13357 in a submerged batch system. Effects of sedimented POME concentration and the initial culture pH on the production of ABE/H were studied. Sedimented POME with 90% v/v (POME90) at pH 5.8 is capable of producing 4.01 g/L ABE with acetone concentration at 1.97 g/L; butanol 1.74 g/L and ethanol 0.3 g/L. The highest concentration of butanol (1.86 g/L) was produced from a culture with initial pH 6.0. The production of hydrogen gas was proportioned to the concentration of POME. The highest hydrogen gas production was at pH 5.5 (31 mL). More than 50% (v/v) of hydrogen gas was produced at different pH except pH 4.5, when only 16% (v/v) or 5 mL of hydrogen was produced.
RUJUKAN/REFERENCES
American Public Health Association (APHA). American Water Works Association and Water Environment Federation. 1992. Standard Method for the examination of water and wastewater. Washington American Public Health Association. 18th edition. USA.
Durre, P. 1998. New insights and novel developments in clostridiaal acetone/butanol/ isopropanol fermentation. Appl Microbiol Biotechnol 49: 639-648.
Jones, D.T. 2001. Applied acetone-bbutanol fermentation. Clostridia Biotechnology and Medical Applications. 125-168.
Jones, D.T. & Woods D.R., 1986. Acetone-butanol-ethanol revisited. Microbiol Rev 50: 484-24.
Jones, D.T. & Woods D.R., 1989. Solvent production in Clostridia Edited by Minton, N.P. & Clarke, D.J., Plenum Press 105-144.
Khaw, T.S., Arbakariya, A. Mohd. Ali, H. & Mohd. Ismail A.K. 1999. Utilization of fermentable sugars from saccharification of palm oil mill effluent (POME) and oil palm empty fruit bunch (OPEFB) fivers for acetone-butanol-ethanol (ABE) fermentation. Proceeding for Seminar on efficient use of palm oil waste as renewable resource for energy and chemical. Bali, Indonesia (5th November 1999).
Lee, T.M., Ayaaki, I., Sadazo, Y. & Kensuke, F. 1995. Production of acetone, butanol and ethanol from palm oil waste by Clostridum saccharoperbutylacetonicum N1-4. Biotechnol. Lett. 17: (6) 649-654.
Monot, F., Engasser, J.M. & Petidemange, H. 1984. Influence of pH and undissociated butyric acid on the production of acetone and butanol in batch cultures of Clostridium acetobutlylicum. Appl. Microbiol. Biotechnol. 19: 422-426.
Volesky, B., Mulchandani, A. & Williams, J. 1981. Biochemical Production of Industrial Solvents (acetone-butanol-ethanol) from Renewable Sources. Annual New York Acad Sci 369: 205-218.
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